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2018b n a liposome microarray normalized data  (DSMZ)


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    DSMZ 2018b n a liposome microarray normalized data
    2018b N A Liposome Microarray Normalized Data, supplied by DSMZ, used in various techniques. Bioz Stars score: 96/100, based on 582 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2018b+n+a+liposome+microarray+normalized+data/HEP-G2/pm35235798-322-90-125
    Average 96 stars, based on 582 article reviews
    2018b n a liposome microarray normalized data - by Bioz Stars, 2026-09
    96/100 stars

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    Bicinchoninic Acid Protein Assay:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1

    Enzyme-linked Immunosorbent Assay:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1

    Microarray:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1

    High Performance Thin Layer Chromatography:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1

    Software:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1

    Modification:

    Article Title: Isoform- and cell-state-specific lipidation of ApoE in astrocytes.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-human ApoE (clone wu e-4) Santa Cruz Biotechnology Cat#sc-53570; RRID:AB_781458 Mouse anti-human ApoA1 (clone B-10) Santa Cruz Biotechnology Cat#sc-376818; RRID:AB_2797313 Rabbit anti-a/b tubulin Cell Signaling Technology Cat# 2148; RRID:AB_2288042 Sheep anti-mouse IgG HRP Sigma-Aldrich Cat# GENA931 Goat anti-rabbit IgG HRP Cell Signaling Technology Cat# 7074; RRID:AB_2099233 Goat biotinylated anti-human ApoE antibody Meridian Life Science Cat# K74180B; RRID:AB_150544 Bacterial and virus strains One ShotTM BL21 StarTM (DE3) Chemically Competent E. coli Invitrogen Cat# C6010-03 Biological samples Human post-mortem brain biopsies Netherlands Brain Bank N/A Chemicals, peptides, and recombinant proteins TCEP Sigma-Aldrich Cat# C4706; CAS:51805-45-9 Benzonase nuclease Millipore Cat# 70746; CAS:9025-65-4 Lysozyme Sigma-Aldrich Cat# 62971; CAS:12650-88-3 Imidazole Sigma-Aldrich Cat# I202; CAS:288-32-4 HEPES Sigma-Aldrich Cat# H3375; CAS:7365-45-9 DTT Biomol Cat# 04010.10; CAS:3483-12-3 IPTG solution Thermo Fisher Scientific Cat# R1171; CAS:367-93-1 Triton X-100 Sigma-Aldrich Cat# T9284; CAS:9036-19-5 NP-40 AppliChem Cat# A1694.0250; CAS:127087-87-0 Sodium deoxycholate AppliChem Cat# 30970; CAS:302-95-4 Protease inhibitor cocktail Roche Cat# 11873580001 Phosphatase inhibitor Sigma-Aldrich Cat# 4906845001 Enhanced chemiluminescence (ECL) Millipore Cat# WBKLS0500 Copper(II) sulfate pentahydrate (CuSO4) Sigma-Aldrich Cat# 12849; CAS:7758-99-8 T0901317 Sigma-Aldrich Cat# T2320; CAS:293754-55-9 Probucol Sigma-Aldrich Cat# P9672; CAS:23288-49-5 BLT-1 Sigma-Aldrich Cat# SML0059; CAS:321673-30-7 Progesterone Sigma-Aldrich Cat# P8783; CAS:57-83-0 A922500 Sigma-Aldrich Cat# A1737; CAS:959122-11-3 Vitronectin StemCell Technologies Cat# 07004 LipidSpot 610 Biotium Cat# 70069 Hoechst 33342 Abcam Cat# ab228551 ER-Tracker Green Invitrogen Cat# E34251 MitoTracker Green Invitrogen Cat# M7514; CAS:201860-17-5 BODIPY FL C5-ceramide Invitrogen Cat# B22650 Streptavidin-coated agarose beads Sigma-Aldrich Cat# S1638 Ni-NTA agarose Qiagen Cat# 30210 Apolipoprotein A1 (ApoA1) Antibodies-online Cat# ABIN377075 Apolipoprotein E (ApoE) Antibodies-online Cat# ABIN3119657 Fatty acid-free BSA Sigma-Aldrich Cat# A3803; CAS:9048-46-8 His-ApoE2 full length/N-terminus Petros et al. (2019) N/A His-ApoE3 full length/N-terminus Petros et al. (2019) N/A (Continued on next page) e1 Cell Reports 38, 110435, March 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER His-ApoE4 full length/N-terminus Petros et al. (2019) N/A sfGFP-ApoE2 full length/N-terminus This paper N/A sfGFP-ApoE3 full length/N-terminus This paper N/A sfGFP-ApoE4 full length/N-terminus This paper N/A Mammalian ApoE2 Petros et al. (2019) N/A Mammalian ApoE3 Petros et al. (2019) N/A Mammalian ApoE4 Petros et al. (2019) N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Fisher Scientific Cat# 23227 Apolipoprotein E Human ELISA Kit Thermo Fisher Scientific Cat# EHAPOE Deposited data Lipidome data of mouse brain, heart, kidney and liver Choi et al., (2018a, 2018b) N/A Liposome microarray normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 RALS normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Lipidomics normalized raw intensities This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 HPTLC normalized data This paper http://dx.doi.org/10.17632/xzxgtrczrh.1 Experimental models: Cell lines Hep-G2 cell line DSMZ Cat# ACC-180; RRID:CVCL_0027 CCF-STTG1 cell line ECACC Cat# 90021502; RRID:CVCL_1118 APOE-KO hiPSC line EBiSC Cat# BIONi010-C-3 APOE2 hiPSC line EBiSC Cat# BIONi010-C-6 APOE3 hiPSC line EBiSC Cat# BIONi010-C-2 APOE4 hiPSC line EBiSC Cat# BIONi010-C-4 Oligonucleotides ApoE forward GAGATCGGCCCACAG GGCCTTAAAGTCGAACAGGCCGTT GAAACC This paper N/A ApoE full-length reverse GAGATCGG CCGATATGGCCTTAATGGTTATCG GACGGGACCGG This paper N/A ApoE amino-terminal reverse GAGAT CGGCCGATATGGCCTTAGCCTGCT TGGTAAACGGCC This paper N/A Software and algorithms (Fiji is just) ImageJ 2.0.0 NIH https://imagej.nih.gov/ij/; RRID:SCR_002285 CellProfiler Image Analysis Software 4.0.7 Broad Institute https://cellprofiler.org/; RRID:SCR_007358 Progenesis QI Nonlinear Dynamics https://www.nonlinear.com/progprogen/qi/ RRID:SCR_018923 R Software 3.5.0 (ggbiplot, RColorBrewer, reshape2 packages) R Core Team https://www.r-project.org Graphpad Prism 9 GraphPad https://www.graphpad.com/ scientific-software/prism/; RRID:SCR_002798 BioRender BioRender https://www.BioRender.com Other RPMI 1640 Medium (ATCC modification) Gibco Cat# A1049101 TeSRE8 medium StemCell Technologies Cat# 05990 ReleasR StemCell Technologies Cat# 05872 (Continued on next page) Cell Reports 38, 110435, March 1, 2022 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Astrocyte Medium ScienCell Cat# 1801 Fetal bovine serum (heat inactivated) Gibco Cat# 10500-064 Methanol Merck Cat# 1060091000; CAS:67-56-1 Chloroform Merck Cat# 1024451000; CAS:67-66-3 Ammonium formate MS-grade Honeywell Fluka Cat# 14266; CAS:540-69-2 Formic acid MS-grade Biosolve Cat# 069141; CAS:64-18-6 Acetonitrile MS-grade Merck Cat# 1000301000; CAS:75-05-8 Cyclohexane Sigma-Aldrich Cat# 227048; CAS:110-82-7 Ethyl acetate Merck Cat# 1096231000; CAS:141-78-6 Hexane Sigma-Aldrich Cat# 296090; CAS:110-54-3 Isopropanol MS-grade Biosolve Cat# 162641; CAS:67-63-0 Acetic acid Merck Cat# 1000621000; CAS:64-19-7 85% ortho-phosphoric acid Merck Cat# 1005731000; CAS:7664-38-2 Ammonium hydroxide solution Sigma-Aldrich Cat# 30501; CAS:1336-21-6 Diethyl ether Thermo Fisher Scientific Cat# 10263230; CAS:60-29-7 Lipids Avanti, Sigma, Atto-Tec This paper – Table S1



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